The inductive effect of bone morphogenetic protein (BMP) on human periodontal fibroblast-like cells in vitro

Bone morphogenetic protein (BMP) has the capability of 1) inducing bone formation in non-bone tissue such as muscle, 2) promoting proliferation and differentiation of human dental pulpal cells, and 3) inducing early formation of both osteodentin and regular tubular dentin. In addition, when BMP is implanted in periodontal defects, it stimulates regeneration of periodontal tissues. In the present study, we sought to explore the mechanisms by which BMP induces these effects, using immunohistochemical methods to investigate periodontal fibroblast-like cells in vitro. Cellular production of fibronectin, endogenous BMP and alkaline phosphatase were measured using image analysis of immunohistochemical reactivity. Periodontal fibroblast-like cells incubated with exogenous BMP showed decreased alkaline phosphatase activity, unchanged fibronectin production, but increased expression of endogenous BMP compared with control specimens incubated without BMP. These data suggest that regenerative as well as inductive effects of BMP on undifferentiated periodontal mesenchymal cells may be mediated through its ability to modulate cellular expression of these substances.