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X-ray microanalysis in cell biology and cell pathology

G M Roomans1, A Von Euler

  • 1Department of Human Anatomy, University of Uppsala, Sweden.

Cell Biology International
|February 1, 1996
PubMed
Summary

Electron probe X-ray microanalysis offers detailed elemental distribution in cells and tissues. Advanced techniques enable accurate analysis of cellular ions, expanding applications in cell biology and pathology.

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Area of Science:

  • Cell Biology
  • Biophysics
  • Pathology

Background:

  • Electron probe X-ray microanalysis (EPXMA) has been a biological tool for 25 years.
  • Progress in EPXMA has focused on specimen preparation and quantitative analysis.
  • EPXMA enables accurate analysis of physiologically important cellular ions.

Purpose of the Study:

  • To review the advancements in EPXMA for biological research.
  • To highlight the application of EPXMA in cell biology and pathology.
  • To discuss specific applications in epithelial ion transport, calcium's role, and cell proliferation.

Main Methods:

  • Specimen preparation, particularly low-temperature techniques.
  • Quantitative analysis methods for elemental distribution.
  • Application of EPXMA to in vitro systems and cell cultures.

Main Results:

  • Improved techniques allow for accurate analysis of cellular ions.
  • EPXMA provides insights into elemental distribution at cellular and tissue levels.
  • In vitro systems and cell cultures broaden EPXMA's applicability.

Conclusions:

  • EPXMA is a valuable technique for elemental analysis in biological samples.
  • Advancements in methodology enhance the accuracy and scope of EPXMA.
  • EPXMA has significant applications in understanding cellular processes and disease states.

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