Ultrafiltrate sampling device for continuous monitoring
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Updated: Nov 3, 2026

Single Step Isolation of Extracellular Vesicles from Large-Volume Samples with a Bifurcated A4F Microfluidic Device
Published on: February 2, 2024
1Department of Biological Psychiatry, University of Groningen, Netherlands.
Abstract:
A light, portable sampling device for the continuous collection and storage of subcutaneous fluid is described. It consists of a hollow-fibre probe for ultrafiltration, a long tube for sample storage and a vacuum tube as driving force. Introduction of a restriction in the flow path allows a constant pulse-free flow as low as 100 nl min-1. The contents of the storage tube reflect the metabolic events that have happened during the 24 h collection period. The ultrafiltrate fluid collected from the subcutaneous tissue is free from large molecules and can be continuously analysed. An application in combination with a bionsensor for the analysis of glucose is described, but the device also allows monitoring of other subcutaneous constituents such as ions, metabolites, drugs and their metabolites.
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