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Striatal cytomegalic neurons containing nitric oxide are associated with experimental perinatal asphyxia: implication
C F Loidl1, F Capani, J J Lopez-Costa
1Instituto de Biología Celular y Neurociencias Eduardo De Robertis, LANAIS-MIE, Facultad de Medicine, Universidad de Buenos Aires.
Abstract:
Neuropathological mechanisms triggered by excitatory aminoacids are known to involve nitric oxide (NO). Neurons containing NO are histochemically reactive to nicotinamide adenine dinucleotide phosphate diaphorase (NADPH-d), which labels NO synthase in CNS. Sprague-Dawley male rats subjected to perinatal asphyxia (PA) at 37 degrees C, and PA plus 15 degrees C hypothermia were evaluated when 6 months old by NADPH-d histochemical reaction. Computarized image analysis was used for quantification of stained sections. NADPH-d neurons in striatum from subsevere and severe PA showed a significant increment in soma size and dendritic process length versus control and hypothermic treated rats. Post-ischemic damage neurons are therefore involved in NO changes induced by PA that may be prevented by hypothermia treatment.