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Isolation and characterization of a functional promoter from Nitrosomonas europaea
A Nejidat1, O Spector, A Abeliovich
1Jacob Blaustein Institute for Desert Research, Ben-Gurion University of the Negev, Israel.
FEMS Microbiology Letters
|March 15, 1996
Abstract:
A functional promoter from the obligate autotrophic ammonia oxidizing bacterium Nitrosomonas europaea was identified by expression in Escherichia coli, using a promoterless reporter gene. Transcription initiation site of this promoter was determined by primer extension analysis. The sequences at -35 and -10 have low similarity to the -10/-35 consensus sequence of known prokaryotic promoters.