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A new monoclonal antibody detecting ochratoxin A at the picogram level
A Gyöngyösi-Horváth1, I Barna-Vetró, L Solti
1Agricultural Biotechnology Center, Gödöllö, Hungary.
Letters in Applied Microbiology
|February 1, 1996
Abstract:
A monoclonal antibody against ochratoxin A was produced after immunization of Balb/c mice with ochratoxin A-BSA. This antibody was of the IgG1 heavy chain subclass with a kappa type light chain. The 50% inhibition value was 0.45 ng ml-1 in a direct competitive ELISA and the detection limit was 42 pg ml-1. This antibody is very specific, cross-reacting only with ochratoxin B (9.3%).