Patterns of degeneration in the external cuneate nucleus after multiple dorsal rhizotomies

Unilateral, intradural dorsal rhizotomies (C3-Cs) were performed on adult rats to study the patterns of synaptic organization of ascending dorsal root fibers in the external cuneate nucleus (ECN). Animals were permitted to survive for periods of time ranging from 3 hours to 12 days. Sham-operated animals presented a morphology indistinguishable from that of normal, unoperated animals. In rhizotomized animals, degeneration was observed ipsilaterally at all survival periods. After postoperative survivals of 3 to 14 hours some terminal boutons displayed clumping and diminution in numbers of synaptic vesicles and, in addition, degeneration myelinated axons were observed at this time. There was considerable degeneration in the neuropil between 24 and 48 hours postoperative. Two forms of degeneration occurred in axons and terminal boutons with comparable frequency: electron lucent degeneration and electron opaque degeneration. Reactive phagocytic glial cells contained degenerated masses, lipoid droplets, lysosome-like structures and myelin fragments. After postoperative survivals of four to six days, lucent and opaque degenerating terminals were less numerous. Neurofilamentous degeneration was observed only occasionally. Unaltered synaptic membrane specializations were present and were usually abutted by glia. At 12 days postoperative, synaptic glomeruli and serial synapses were not seen. Invaginating dendritic spines were rarely seen. Bouton populations that remained unualtered were: small (0.3-3.0 micron) boutons that contact dendritic shafts and somata, nodal synaptic boutons and boutons containing granular vesicles (80-100 nm).