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Related Experiment Videos

Minimal change-like glomerular alterations induced by a human plasma factor

P K Cheung1, P A Klok, W W Bakker

  • 1Department of Pathology, University of Groningen, The Netherlands.

Nephron
|January 1, 1996
PubMed
Summary

A specific plasma protease, 100 KF, significantly increases protein leakage in kidneys, mimicking minimal change disease (MCD). This factor impairs glomerular polyanion and ecto-ATPase, suggesting its role in MCD pathogenesis.

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Area of Science:

  • Nephrology
  • Pathophysiology
  • Biochemistry

Background:

  • Minimal change disease (MCD) pathogenesis is linked to circulating factors.
  • A plasma protease, termed 100 KF, has been implicated in MCD.
  • Previous in vitro studies suggested 100 KF induces MCD-like kidney alterations.

Purpose of the Study:

  • To investigate the in vivo effect of 100 KF on kidney permeability and protein leakage.
  • To assess the impact of 100 KF on glomerular polyanion (GPA) and glomerular ecto-ATPase.
  • To determine if 100 KF can induce minimal change disease-like lesions in ex vivo perfused rat kidneys.

Main Methods:

  • Ex vivo perfusion of rat kidneys with either native 100 KF or a control factor.
  • Collection of urine samples for measurement of total protein, IgG, and albumin.

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  • Histochemical and immunohistochemical staining of kidney sections for GPA and glomerular ecto-ATPase, quantified by image analysis.

    • Main Results:

    • Perfusion with 100 KF significantly increased urinary protein leakage compared to control (150.0 vs. 33.2 µg/min).
    • The IgG/albumin ratio decreased significantly after 100 KF perfusion (12.0% vs. 26.9%).
    • 100 KF perfusion led to significant loss of GPA (57.3 vs. 98.4 arbitrary units) and reduced glomerular ecto-ATPase expression (28.7 vs. 79.5 arbitrary units).

    Conclusions:

    • The 100 KF factor induces minimal change disease-like glomerular lesions in ex vivo perfused rat kidneys.
    • Increased plasma protein permeability is associated with GPA loss and reduced glomerular ecto-ATPase expression.
    • This human plasma constituent, 100 KF, is a potential key factor in the pathogenesis of minimal change disease.