Bilateral biochemical and biophysical retinal changes after unilateral ocular trauma in the mouse

The bilateral response of the mouse retinae after puncture and insertion of iron wire in one eye was studied by electrophoretic analysis of protein changes and free radicals quantitation at 4, 15 and 42 days after surgery. The effect of cyclosporine was also studied. Several low molecular weight (< 24 kDa) polypeptide subunits were detected in both the traumatized and contralateral eyes of experimental animals. Free radical levels rose in both traumatized and contralateral eyes although the extent was much greater in the former. Both biochemical and biophysical assays showed that treatment with cyclosporine was effective in suppressing the responses in retinal proteins and free radicals only for up to 15 days. These results substantiated our earlier report that unilateral injury to one eye with retention of the foreign body affects both eyes in the mouse.