Calcium-dependent after-hyperpolarization in dissociated bullfrog sympathetic neurons

Whole-cell recordings were made from dissociated bullfrog sympathetic neurons. Tetraethylammonium (30 mM) and apamin (100 nM) were added to the superfusate to eliminate the known calcium-activated potassium currents termed Ic and IAHP. Under these conditions, the action potential carried by calcium ions was followed by a prolonged (10-60 s) after-hyperpolarization. A current component (IAC) underlying the after-hyperpolarization was eliminated by barium (2 mM) and showed voltage-dependence identical to that of a M-type potassium current. I concluded that the after-hyperpolarization is caused not only by IAHP but also by the calcium-dependent potentiation of M-current.