Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Phage display of proteins

I S Dunn1

  • 1Queensland Cancer Fund Research Unit, Department of Patholog,y Medical School, University of Queensland, Herston 4006, Brisbane, Queensland, Australia. i.dunn@mailbox.uq.oz.au

Current Opinion in Biotechnology
|October 1, 1996
PubMed
Summary

Phage display technology advances by integrating rational protein design with selection. New bacteriophage systems offer improved methods for selecting surface-displayed proteins and cDNA clones.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Strategies to overcome obstacles to successful immunotherapy of melanoma.

International journal of immunopathology and pharmacology·2008
Same author

Tracking membrane and secretory immunoglobulin alpha heavy chain mRNA variation during B-cell differentiation by real-time quantitative polymerase chain reaction.

Immunology and cell biology·2001
Same author

A novel autocrine pathway of tumor escape from immune recognition: melanoma cell lines produce a soluble protein that diminishes expression of the gene encoding the melanocyte lineage melan-A/MART-1 antigen through down-modulation of its promoter.

Journal of immunology (Baltimore, Md. : 1950)·2001
Same author

Quantifiable analysis of human immunoglobulin heavy chain class-switch recombination to all isotypes.

Journal of immunological methods·2000
Same author

Expression and localization of the retinoblastoma gene during radiation-induced apoptosis in neonatal rat kidney.

Experimental cell research·1997
Same author

Tumor selectivity and transcriptional activation by azelaic bishydroxamic acid in human melanocytic cells.

Biochemical pharmacology·1997

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Protein Engineering

Background:

  • Phage display remains a vital technology for diverse applications.
  • Recent progress involves combining rational protein design with display selection.

Purpose of the Study:

  • To highlight advancements in phage display technology.
  • To introduce novel bacteriophage surface expression systems.

Main Methods:

  • Utilizing filamentous phage display systems.
  • Developing and employing alternative bacteriophage surface expression systems.

Main Results:

  • Successful integration of rational design principles with phage display selection.
  • Development of new phage display systems beyond filamentous phage.

Conclusions:

  • Phage display technology continues to evolve with enhanced capabilities.
  • New systems provide valuable tools for selecting surface-displayed proteins and cDNA clones.

Related Experiment Videos