Genetic and biochemical probes for protein-protein interactions

The two-hybrid system is a powerful approach for examining protein-protein interactions. Recently, the utility of the system has been extended to include the genome-wide mapping of protein-protein interactions and the identification of peptide inhibitors of protein interactions. In addition, immunophilins and their chemical ligands are providing useful reagents for generating conditional protein-protein interactions in vivo to dissect intracellular signaling pathways.