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Secretion of recombinant proteins by Gram-negative bacteria
1Building 30, Room 313, National Institutes of Health/National Institute of Dental Research, Bethesda, MD 20892-4350, USA. msandkvist@yoda.nidr.nih.gov
Current Opinion in Biotechnology
|October 1, 1996
Summary
Understanding bacterial protein translocation is advancing, enabling recombinant protein secretion. Further research is needed for precise manipulation of secretion systems to export non-native proteins.
Area of Science:
- Microbiology
- Molecular Biology
- Biochemistry
Background:
- Significant progress in understanding bacterial protein translocation mechanisms.
- Recombinant protein secretion has seen successful empirical and knowledge-based attempts.
Purpose of the Study:
- To deepen the understanding of bacterial membrane-translocation mechanisms.
- To enable predictable manipulation of protein secretion systems.
Main Methods:
- Review of recent molecular mechanism studies.
- Analysis of empirical efforts in recombinant protein secretion.
Main Results:
- Knowledge of protein translocation has improved significantly.
- Successful, though not fully predictable, secretion of some recombinant proteins achieved.
Conclusions:
- Further in-depth understanding of membrane translocation is crucial.
- Predictable secretion of non-native extracellular proteins requires more research into secretion systems.