Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Metabolic engineering of polyketide biosynthesis

C L Hershberger1

  • 1Lilly Corporate Center, 3224 Eli Lilly & Co., Indianapolis, IN 46285, USA. hershberger_charles_l@lilly.com

Current Opinion in Biotechnology
|October 1, 1996
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Patents and literature.

Applied biochemistry and biotechnology·2013
Same author

Purification, characterization, cDNA cloning and expression of a novel ketoreductase from Zygosaccharomyces rouxii.

European journal of biochemistry·2000
Same author

Mapping the pro-region of carboxypeptidase B by protein engineering. Cloning, overexpression, and mutagenesis of the porcine proenzyme.

The Journal of biological chemistry·1999
Same author

Activation of the human estrogen receptor by estrogenic and antiestrogenic compounds in Saccharomyces cerevisiae: a positive selection system.

Gene·1996
Same author

A cloned gene for human transferrin.

Annals of the New York Academy of Sciences·1992
Same author

Homology between proteins controlling Streptomyces fradiae tylosin resistance and ATP-binding transport.

Gene·1991

New polyketides were generated using advancements in metabolic pathway genes, host strains, and recombinant DNA technology. This combinatorial biology approach enabled rational design and biosynthesis of novel molecules.

Area of Science:

  • Biotechnology
  • Synthetic Biology
  • Metabolic Engineering

Background:

  • Polyketides are a diverse class of natural products with significant therapeutic potential.
  • Previous limitations in polyketide synthesis hindered the exploration of novel analogs.

Purpose of the Study:

  • To develop a platform for the rational design and biosynthesis of novel polyketides.
  • To leverage advancements in genetic engineering and combinatorial biology for molecule discovery.

Main Methods:

  • Utilized cloned genes from various metabolic pathways.
  • Employed a genetically defined host strain for polyketide production.
  • Applied recombinant DNA technology for gene modification and recombination.
  • Implemented combinatorial biology strategies for large-scale molecule generation.

Related Experiment Videos

Main Results:

  • Successfully generated a large number of novel polyketide molecules.
  • Demonstrated the feasibility of rational molecular design through genetic manipulation.
  • Established a robust system for combinatorial biosynthesis of polyketides.

Conclusions:

  • The integration of genetic tools and combinatorial biology enables efficient generation of diverse polyketide libraries.
  • This approach accelerates the discovery of new molecules with potential pharmaceutical applications.
  • Future research can focus on further optimizing pathways and exploring broader chemical diversity.