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Related Experiment Videos

A volume-activated cell sorter

E Menke, E Kordwig, P Stuhlmüller

    The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
    |July 1, 1977
    PubMed
    Summary

    A novel cell sorter utilizes Coulter volume detector signals for high-throughput cell sorting. This system achieves over 97% purity and 80% viability, processing up to 2000 cells per second.

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    Area of Science:

    • Biotechnology
    • Cell Biology
    • Analytical Chemistry

    Background:

    • Cell sorting is crucial for biological research and diagnostics.
    • Traditional methods face challenges with signal distortion and processing speed.
    • Developing advanced cell sorters is essential for improving efficiency and accuracy.

    Purpose of the Study:

    • To develop a new cell sorter activated by Coulter orifice resistance pulses.
    • To overcome signal distortion issues caused by electric droplet formation and charging.
    • To evaluate the performance of the developed cell sorter using various cell types.

    Main Methods:

    • A cell sorter was designed, integrating a Coulter orifice (volume detector).
    • Special shielding and grounding techniques were employed to prevent signal distortion.
    • Experiments were conducted using standard beads and primary leukocytes.
    • Cell sorting performance was assessed based on processing rate, purity, and cell viability.

    Main Results:

    • The developed cell sorter effectively utilizes Coulter volume signals.
    • Signal distortion was minimized through specialized shielding and grounding.
    • Maximal processing rates reached 2000 cells/sec.
    • Purity of sorted fractions exceeded 97%, with cell viability above 80%.

    Conclusions:

    • The developed Coulter-activated cell sorter is a high-performance system.
    • It offers a reliable method for rapid and pure cell fraction isolation.
    • The system demonstrates significant potential for various cell analysis applications.

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