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Related Experiment Videos

Steroid sulfotransferases

V Luu-The1, F Bernier, I Dufort

  • 1Medical Research Council Group in Molecular Endocrinology, CHUL Research Center, Quebec, Canada.

The Journal of Endocrinology
|September 1, 1996
PubMed
Summary
This summary is machine-generated.

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Human dehydroepiandrosterone sulfotransferase (DHEA-ST) gene promoter activity is stimulated by estradiol. This study cloned and sequenced DHEA-ST cDNA, revealing identical adrenal and liver forms, and identified alternative promoters for multiple mRNA species.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Genetics

Background:

  • Sulfotransferases are crucial enzymes catalyzing the sulfonation of various steroids and xenobiotics.
  • Human dehydroepiandrosterone sulfotransferase (DHEA-ST) plays a role in steroid metabolism.
  • Understanding the regulation and diversity of sulfotransferase genes is essential for comprehending their physiological functions.

Purpose of the Study:

  • To clone and sequence human adrenal DHEA-ST cDNA to compare it with the liver form.
  • To investigate the regulatory elements of the DHEA-ST gene promoter, particularly its response to estradiol.
  • To characterize the structural diversity and gene organization of human sulfotransferases, including estrogen and phenol sulfotransferases.

Main Methods:

  • RNA blot analysis to identify DHEA-ST mRNA species.

Related Experiment Videos

  • Cloning and sequencing of DHEA-ST cDNA from a human adrenal library.
  • Reporter gene assays (chloramphenicol acetyltransferase - CAT) to study promoter activity.
  • Sequence analysis of cloned cDNAs and genes.

    • Main Results:

    • Two DHEA-ST mRNA species (1.3 and 1.8 kb) were detected in liver and adrenals.
    • Human adrenal and liver DHEA-ST cDNAs are nearly identical, differing by only one nucleotide.
    • DHEA-ST gene promoter activity is stimulated by estradiol (E2) and inhibited by the anti-estrogen EM-139.
    • Identification of multiple mRNA species for estrogen sulfotransferase (hEST/STM) and phenol sulfotransferase (STP) genes, arising from alternative promoters and differing 5'-untranslated regions.

    Conclusions:

    • The DHEA-ST expressed in human adrenals and liver is essentially identical.
    • Estradiol positively regulates DHEA-ST gene expression via its promoter.
    • The STM and STP genes exhibit complex transcriptional regulation, generating diverse mRNA isoforms through alternative promoter usage, contributing to functional specialization.