Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Computational method to predict mitochondrially imported proteins and their targeting sequences

M G Claros1, P Vincens

  • 1Laboratoire de Génétique Moléculaire, CNRS URA 1302, Ecole Normale Supérieure, Paris, France. claros@uma.es or claros@cica.es

European Journal of Biochemistry
|November 1, 1996
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Expression-based, consistent biomarkers for prognosis and diagnosis in lung cancer.

Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·2020
Same author

Genomic characterization and expression analysis of four apolipoprotein A-IV paralogs in Senegalese sole (Solea senegalensis Kaup).

Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology·2015
Same author

Genome-wide gene expression analysis during Solea sp. embryo-larval development.

Communications in agricultural and applied biological sciences·2014
Same author

Evidence for an operative glutamine translocator in chloroplasts from maritime pine (Pinus pinaster Ait.) cotyledons.

Plant biology (Stuttgart, Germany)·2010
Same author

MOSAIC: segmenting multiple aligned DNA sequences.

Bioinformatics (Oxford, England)·2001
Same author

Subunit II of cytochrome c oxidase in Chlamydomonad algae is a heterodimer encoded by two independent nuclear genes.

The Journal of biological chemistry·2000

Researchers developed a computational method to predict mitochondrial proteins using amino acid sequences. This method accurately identifies proteins targeted for import into mitochondria, aiding in the analysis of cellular functions.

Area of Science:

  • Biochemistry
  • Computational Biology
  • Molecular Biology

Background:

  • Mitochondria are vital organelles requiring specific protein import mechanisms.
  • Protein targeting information is encoded within protein sequences and structures.
  • Predicting mitochondrial protein localization is crucial for understanding cellular processes.

Purpose of the Study:

  • To develop a computational method for predicting mitochondrially imported proteins.
  • To analyze sequence and structural parameters for accurate protein targeting prediction.
  • To identify potential mitochondrial proteins in yeast open reading frames.

Main Methods:

  • Discriminant analysis utilizing 47 parameters.
  • Analysis of a large dataset of mitochondrial proteins from the SwissProt database.

Related Experiment Videos

  • Development of a computational prediction tool based on amino acid sequence.

    • Main Results:

    • The developed computational method achieved 75-97% accuracy in predicting mitochondrial protein import based on amino acid sequence alone.
    • Mitochondrial-targeting sequences were predicted in 76-94% of analyzed precursor proteins.
    • A significant number of unknown yeast open reading frames were identified as potential mitochondrial proteins, often found in clusters.

    Conclusions:

    • A robust computational approach for predicting mitochondrial protein localization has been established.
    • The method demonstrates high accuracy, relying primarily on amino acid sequence data.
    • This tool has practical applications in identifying novel mitochondrial proteins and understanding yeast proteomes.