Relation of intromissions to the female's postejaculatory refractory period in rats

The objectives of this study were to investigate the temporal aspects of female sexual behavior during single and multiple ejaculatory tests. Females were tested in a two-compartment chamber where they could escape from the male following sexual contacts. In Experiment 1, correlation analysis showed that the number of intromissions received by the female over 3 ejaculatory series was positively correlated with the female's postejaculatory refractory period (PER). In Experiment 2, females receiving 2-4 intromissions before ejaculation had a PER that did not differ from those receiving 5-15 (average 10) intromissions preceding ejaculation. However, if the male ejaculated on the first or second intromission, the female's PER was significantly shorter than the other groups and did not differ from her return latency after an intromission without ejaculation. Females receiving 24-31 intromissions preceding ejaculation exhibited the longest PER. Analysis revealed that the number of intromissions received by females before ejaculation was positively correlated with the female's PER. We concluded that the male's ejaculatory reflex, seminal emission, and postejaculatory behavior alone without at least 2 preceding intromissions were not sufficient to induce a female's PER comparable to that seen after an ejaculation during normal copulation. In addition, the number of intromissions received by the female preceding ejaculation was positively correlated with the female's PER if the range of intromission frequency was large enough.