Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Nitric oxide, a physiological modulator of mitochondrial function

S Okada1, Y Takehara, M Yabuki

  • 1Department of Cell Chemistry, Okayama University Medical School, Japan.

Physiological Chemistry and Physics and Medical NMR
|January 1, 1996
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Erythroid and megakaryocytic differentiation of K562 erythroleukemic cells by monochloramine.

Free radical research·2013
Same author

Carotid intima-media thickness and cerebral white matter lesions are more advanced in acute ischemic stroke patients with renal dysfunction.

Clinical nephrology·2011
Same author

The effectiveness of packed therapy with three drugs in Helicobacter pylori eradication in Japan.

Methods and findings in experimental and clinical pharmacology·2010
Same author

Change in environmental bacterial flora in a new hospital building.

The Journal of hospital infection·2009
Same author

Immunoregulation and apoptosis induction of nitric oxide in the human mixed lymphocytes culture.

Transplantation proceedings·2006
Same author

Evaluation of acrosomal status of bovine spermatozoa using concanavalin a lectin.

Theriogenology·2006

Nitric oxide (NO) inhibits mitochondrial functions like respiration and ATP synthesis in a dose-dependent manner. This inhibition is reversible and influenced by oxygen levels, suggesting NO

Area of Science:

  • Mitochondrial physiology
  • Biochemistry
  • Cellular respiration

Background:

  • Mitochondria are crucial for cellular energy production.
  • Nitric oxide (NO) is a signaling molecule with diverse physiological roles.
  • The interaction between NO and mitochondrial function under varying oxygen conditions requires further elucidation.

Purpose of the Study:

  • To investigate the impact of nitric oxide (NO) on mitochondrial energy transfer reactions.
  • To determine the influence of varying oxygen tensions on NO's effects on mitochondrial function.
  • To explore the reversibility and kinetics of NO-mediated inhibition of mitochondrial processes.

Main Methods:

  • Mitochondrial suspensions were prepared.
  • Mitochondrial respiration, ATP synthesis, and membrane potential were measured.

Related Experiment Videos

  • Nitric oxide (NO) was introduced under controlled oxygen tensions.
  • NO concentration and disappearance were assessed using methemoglobin formation and Electron Paramagnetic Resonance (EPR) methods.

    • Main Results:

    • Nitric oxide (NO) inhibited mitochondrial respiration, ATP synthesis, and membrane potential regulation in a concentration-dependent manner.
    • NO also inhibited uncoupled respiration, with the effect being dependent on oxygen concentration.
    • The inhibited mitochondrial functions showed complete recovery over time after NO addition.
    • NO rapidly disappeared from the medium, with faster clearance at higher oxygen tensions.

    Conclusions:

    • Nitric oxide (NO) significantly impacts mitochondrial energy transfer reactions.
    • Oxygen tension modulates the inhibitory effects of NO on mitochondria.
    • NO's rapid disappearance and reversible inhibition suggest a regulatory role, particularly under low oxygen conditions.