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Related Experiment Videos

Solid phase microextraction for quantitative headspace sampling of apple volatiles

A J Matich1, D D Rowan, N H Banks

  • 1Horticulture and Food Research Institute of New Zealand Ltd., Palmerston North, New Zealand.

Analytical Chemistry
|December 1, 1996
PubMed
Summary
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Solid phase microextraction (SPME) is useful for qualitative analysis of apple volatiles. However, slow equilibration of high molecular weight compounds like alpha-farnesene limits its quantitative accuracy in cool storage studies.

Area of Science:

  • Food Science
  • Analytical Chemistry
  • Horticulture

Background:

  • Cool storage of apples can lead to superficial scald, a disorder linked to alpha-farnesene production.
  • Accurate quantification of volatile compounds is crucial for understanding apple storage disorders.

Purpose of the Study:

  • To evaluate solid phase microextraction (SPME) for quantifying aroma volatile production in Granny Smith apples during cool storage.
  • To specifically assess the quantification of alpha-farnesene, a key compound in superficial scald.

Main Methods:

  • Comparison of SPME with solid phase extraction (SPE) for volatile analysis.
  • Investigation of equilibration times for high and low molecular weight volatiles using SPME.
  • Use of a model system with squalane to study volatile adsorption.

Related Experiment Videos

  • Analysis of factors affecting alpha-farnesene adsorption, including air movement and distance in static headspace systems.

    • Main Results:

    • SPME showed greater adsorption of high molecular weight volatiles (e.g., alpha-farnesene) compared to SPE.
    • High molecular weight volatiles exhibited slow equilibration (over 90 min) with SPME, unlike low molecular weight volatiles (within 5 min).
    • Alpha-farnesene adsorption by SPME increased with air movement but decreased with distance in static headspace systems.

    Conclusions:

    • SPME is suitable for rapid, qualitative analysis of apple headspace volatiles.
    • The slow equilibration of high molecular weight volatiles with SPME restricts its quantitative application in complex systems like cool-stored apples.