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Bafilomycin A1 induces apoptosis in PC12 cells independently of intracellular pH
K Kinoshita1, T Waritani, M Noto
1Department of Biochemistry, Faculty of Pharmaceutical Sciences, Kanazawa University, Ishikawa, Japan.
FEBS Letters
|November 25, 1996
Abstract:
PC12 cells growth-arrested with bafilomycin A1 died showing apoptotic chromatin condensation in the nuclei. The bafilomycin A1-induced chromatin condensation was preceded by neurite outgrowth (NOG), required higher concentrations of bafilomycin A1 than NOG, and was suppressed by cycloheximide and aurintricarboxylic acid. NH4Cl (10 mM), another acidotropic pH perturbing agent, neither induced apoptotic chromatin condensation by itself nor suppressed that induced by bafilomycin A1, suggesting that bafilomycin A1-induced apoptosis occurs independently of intracellular pH in PC12 cells.