Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Hierarchic T-cell help to non-linked B-cell epitopes

N H Brons1, A Blaich, K H Wiesmüller

  • 1Laboratorie National de Santé, Luxembourg, Germany.

Scandinavian Journal of Immunology
|November 1, 1996
PubMed
Summary

Antibody production against peptides requires T helper cell epitopes. Adding a T-cell epitope significantly increased immunogenicity, with optimal epitopes identified by their self-immunogenicity for enhanced antibody responses.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Sphingolipids in Emotional Well-Being.

Journal of neurochemistry·2026
Same author

Serelaxin activates eNOS, suppresses inflammation, attenuates developmental delay and improves cognitive functions of neonatal rats after germinal matrix hemorrhage.

Scientific reports·2020
Same author

Individual differences in inflammatory and oxidative mechanisms of stress-related mood disorders.

Frontiers in neuroendocrinology·2019
Same author

Molecular surveillance of measles and rubella in the WHO European Region: new challenges in the elimination phase.

Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases·2017
Same author

Aberrant splicing of the tumor suppressor CYLD promotes the development of chronic lymphocytic leukemia via sustained NF-κB signaling.

Leukemia·2017
Same author

Challenges of measles and rubella laboratory diagnostic in the era of elimination.

Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases·2017

Area of Science:

  • Immunology
  • Vaccine Development
  • Molecular Biology

Background:

  • Antibody induction against peptide antigens necessitates T helper cell epitope presence.
  • Measles virus (MV) fusion (F) protein peptides elicit low antibody responses without T-cell help.

Purpose of the Study:

  • To investigate the role of T-cell epitopes in enhancing peptide immunogenicity.
  • To identify characteristics of T-cell epitopes that confer optimal help to B-cell epitopes.

Main Methods:

  • Co-immunization of mice with measles virus (MV) fusion (F) protein peptides and various T-cell epitopes.
  • Assessment of antibody responses against B-cell epitopes in the presence of different T-cell epitopes.
  • Correlation analysis between T-cell epitope immunogenicity and its ability to provide T-cell help.

Related Experiment Videos

Main Results:

  • Co-immunization with a T-cell epitope increased peptide immunogenicity from <10% to >60%.
  • Immunodominant T-cell epitopes were more effective helpers than subdominant or cryptic ones.
  • T-cell epitope helper capacity correlated directly with its own immunogenicity.

Conclusions:

  • A hierarchy exists for both B-cell epitope help requirements and T-cell epitope helper capabilities.
  • Optimal T-cell epitopes, identifiable by high self-immunogenicity, maximize help for B-cell epitopes across MHC class II alleles.
  • This strategy successfully induced cross-reactive antibodies against measles virus (MV).