Recent advances in the diagnosis of cytomegalovirus infection

Herpesviruses are highly disseminated in nature, and nearly 100 Herpesviruses have been at least partially characterized. Seven Herpesviruses have been isolated so far from humans and one has recently been detected by sequence analysis. Consequently, the diagnosis of human Herpesvirus infection is based on eight different viruses. The diagnosis of a Herpesvirus infection can be achieved through the direct demonstration of the presence of the virus or its components (mainly nucleic acids and antigens) in pathological materials or indirectly through serology. In recent years much progress has been made in both directions and thanks to the detailed study of the viral genomes and their antigenic gene products and by the determination of the immune reactivity against individual antigenic polypeptides in different clinical settings. Progress in the direct detection of the viruses in pathological materials mainly regards the production of specific probes and their use with or without DNA amplification for the detection of viral genomes. Serological advances are mainly linked to the production, characterization and use of specific viral antigens by DNA recombinant procedures and by the production and use of synthetic peptides acting as good antigenic epitopes.