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Related Experiment Videos

Carbohydrate chromatography: towards yoctomole sensitivity

M J Davies1, E F Hounsell

  • 1Department of Biochemistry and Molecular Biology, University College London, UK.

Biomedical Chromatography : BMC
|November 1, 1996
PubMed
Summary

Analyzing complex carbohydrates using HPLC or capillary electrophoresis (CE) is challenging. Recent advances in separation and detection methods now allow for highly sensitive detection of oligosaccharides.

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Area of Science:

  • Analytical Chemistry
  • Biochemistry

Background:

  • Complex carbohydrate analysis presents challenges due to structural diversity and poor chromatographic resolution.
  • The non-chromogenic nature of carbohydrates limits sensitivity in UV detection, and refractive index detection is unsuitable for sensitive analyses.

Purpose of the Study:

  • To review significant advances in carbohydrate separation and detection methods.
  • To highlight potential future developments in the field.

Main Methods:

  • High-performance liquid chromatography (HPLC)
  • Capillary electrophoresis (CE)
  • Electrochemical detection
  • Fluorescence detection (after labelling)
  • Laser-induced fluorescence detection

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Main Results:

  • Conventional HPLC and CE with electrochemical or fluorescence detection can now readily detect 200-300 fmol of oligosaccharides.
  • Miniaturized CE systems coupled with laser-induced fluorescence achieve ultra-high sensitivity, detecting as little as 100 yoctomoles.

Conclusions:

  • Significant progress has been made in sensitive carbohydrate analysis over the last five years.
  • Future developments in miniaturized systems and advanced detection techniques promise even greater sensitivity and resolution for complex carbohydrate analysis.