Presence of casein immunoreactive epitopes in molluscs, fish and frog

The presence of a group of peptides derived from milk proteins (caseins) was examined by immunocytochemistry in various tissues from invertebrates and lower vertebrates. Phagocytic hemocytes from different species of molluscs, and cells located in the intestine wall or in related glands of invertebrates and lower vertebrates showed immunoreactivity to antibodies to whole casein and related fragments. Several functional tests (cell migration, inhibition test, phagocytosis) using these peptides were performed on the mollusc hemocytes. Only ovine caseinoglycopeptide was able to increase the phagocytic activity of the hemocytes towards bacteria.