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Study of oxidative-stress in rotavirus infected infant mice
C P Sodhi1, R Katyal, S V Rana
1Department of Gastroenterology, Postgraduate Institute of Medical Education & Research, Chandigarh.
Abstract:
Rotavirus induced diarrhoea was investigated in neonatal mice. Assessment of oxidative/antioxidative profile was the mechanistic approach to study the nature of injury. Neonatal mice (NMRI strain) were infected orally with the homologous strain of (EB) rotavirus (serotype 3). The peak severity of rotavirus infection was attained on the third day post infection. The whole small intestine of neonatal mice on day 3 post infection was homogenized and analysed for oxidative/antioxidative profile. Glutathione and related thiols were significantly declined in rotavirus infected group. Superoxide dismutase, glutathione peroxidase and glutathione-S-transferase (1-chloro 2, 4 dinitrobenzene) activities were also decreased in the rotavirus infected group. The activities of glutathione reductase and glutathione-S-transferase (ethacrynic acid) however were elevated with rotavirus infection in comparison to the control group. Similarly, ADP-FeCI3, NADPH induced lipid peroxidation was elevated with rotavirus infection. Thus the altered oxidative/antioxidative profile indicated the presence of oxidative stress in the rotavirus infected group and can be postulated to have a prominent role in the pathogenesis of the disease.
Insights
Rotavirus infection in neonatal mice causes significant oxidative stress, indicated by decreased antioxidant levels and increased lipid peroxidation. This oxidative imbalance plays a key role in the disease
Area of Science:
- Gastroenterology
- Pediatric Infectious Diseases
- Biochemistry
Background:
- Rotavirus is a leading cause of severe diarrhea in infants globally.
- Understanding the molecular mechanisms of rotavirus pathogenesis is crucial for developing effective treatments.
- Oxidative stress is implicated in various infectious diseases, but its role in rotavirus-induced enteritis requires further elucidation.
Purpose of the Study:
- To investigate the role of oxidative and antioxidative profiles in rotavirus-induced diarrhea in neonatal mice.
- To elucidate the mechanistic basis of rotavirus-induced intestinal injury.
Main Methods:
- Neonatal mice were orally infected with rotavirus (serotype 3).
- Small intestines were analyzed on day 3 post-infection for oxidative/antioxidative markers.
- Assessed levels of glutathione, related thiols, and activities of key antioxidant enzymes.
- Measured lipid peroxidation using ADP-FeCl3 and NADPH induction.
Main Results:
- Rotavirus infection significantly depleted glutathione and related thiols.
- Activities of superoxide dismutase, glutathione peroxidase, and glutathione-S-transferase (CDNB) were decreased.
- Elevated activities of glutathione reductase and glutathione-S-transferase (ECA) were observed.
- Increased lipid peroxidation was evident in infected mice.
Conclusions:
- Rotavirus infection in neonatal mice induces a state of significant oxidative stress.
- The observed alterations in the oxidative/antioxidative balance are strongly implicated in the pathogenesis of rotavirus-induced enteritis.
- Targeting oxidative stress pathways may offer a therapeutic strategy for rotavirus infections.