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Updated: Jul 16, 2026

Free Radicals in Chemical Biology: from Chemical Behavior to Biomarker Development
Published on: April 15, 2013
Enzymic determinations with rotating bioreactors and continuous-flow-stopped-flow processing. Determination of
B López Ruiz1, M Sánchez-Cabezudo, H A Mottola
1Sección Departamental de Química Analítica, Facultad de Farmacia, Universidad Complutense, Madrid, Spain.
Abstract:
The neuromuscular blocking agent, succinylcholine, and the neurotransmitter, acetylcholine, have been determined in pharmaceutical preparations utilizing a sensing unit comprised of a rotating bioreactor and an amperometric detector. The bioreactor consisted of a rotating disk made of Teflon (1 cm id) with cholinesterase (EC 3.1.1.8) and choline oxidase (EC 1.1.3.17) co-immobilized on the top surface of the disk. Amperometric detection of H2O2 and initial rate measurements were employed for quantification.
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