Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Nuclear localization activity of phytochrome B

K Sakamoto1, A Nagatani

  • 1Laboratory for Photoperception and Signal Transduction, Institute of Physical and Chemical Research (RIKEN), Saitama, Japan.

The Plant Journal : for Cell and Molecular Biology
|November 1, 1996
PubMed
Summary

Plant phytochrome B (phyB) is a light receptor that moves into the nucleus. Its nuclear localization is light-dependent, impacting plant photomorphogenesis.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Spatial distribution of phytochromes.

Journal of plant research·2016
Same author

The phototropin family of photoreceptors.

The Plant cell·2001
Same author

Arabidopsis genomes uncoupled 5 (GUN5) mutant reveals the involvement of Mg-chelatase H subunit in plastid-to-nucleus signal transduction.

Proceedings of the National Academy of Sciences of the United States of America·2001
Same author

Light-induced nuclear translocation of endogenous pea phytochrome A visualized by immunocytochemical procedures.

The Plant cell·2000
Same author

Isolation and characterization of an Arabidopsis mutant, fireworks (fiw), which exhibits premature cessation of inflorescence growth and early leaf senescence.

Plant & cell physiology·2000
Same author

[Phytochrome and plant responses to low fluence light].

Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme·1999

Area of Science:

  • Plant biology
  • Molecular biology
  • Photoreceptor research

Background:

  • Phytochromes are key red/far-red light photoreceptors regulating plant development.
  • The signal transduction pathway of phytochromes remains largely unknown.
  • Phytochrome B (phyB) is a well-characterized phytochrome family member in Arabidopsis.

Purpose of the Study:

  • To investigate the subcellular localization of phytochrome B (phyB) in Arabidopsis.
  • To determine if phyB translocates to the nucleus and if this localization is light-dependent.

Main Methods:

  • Generation of transgenic Arabidopsis expressing a phyB-GUS fusion protein to assess nuclear localization signals.
  • Isolation of nuclei from light-grown wild-type Arabidopsis leaves for immunoblot analysis.
  • Immunocytochemical analysis of protoplasts to confirm nuclear phyB presence.
  • Examination of phyB levels in isolated nuclei under different light conditions.

Main Results:

  • A phyB C-terminal fragment fused to GUS showed nuclear localization, confirming functional nuclear localization signals.
  • Endogenous phyB was detected in isolated nuclei from light-grown wild-type Arabidopsis.
  • Nuclear phyB levels decreased upon dark adaptation, with accelerated reduction after far-red light exposure.
  • Nuclear localization of phyB is demonstrated to be light-dependent.

Conclusions:

  • Phytochrome B (phyB) is actively localized to the nucleus in Arabidopsis.
  • The nuclear localization of phyB is regulated by light signals.
  • These findings provide insights into the light-dependent signal transduction mechanism of phytochrome B.

Related Experiment Videos