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Biodegradation of dimethylsilanediol in soils

C L Sabourin1, J C Carpenter, T K Leib

  • 1Environmental Laboratory, General Electric Corporate Research and Development, Schenectady, New York 12301, USA. sabourin@crd.ge.com

Applied and Environmental Microbiology
|December 1, 1996
PubMed
Summary

Dimethylsilanediol, a polydimethylsiloxane monomer, undergoes biodegradation in soils. Microorganisms like Fusarium oxysporum and Arthrobacter sp. were found to cometabolize this compound, demonstrating its environmental breakdown potential.

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Area of Science:

  • Environmental Science
  • Microbiology
  • Biochemistry

Background:

  • Polydimethylsiloxane (PDMS) hydrolysis in soil yields dimethylsilanediol.
  • The environmental fate and biodegradation pathways of dimethylsilanediol are not fully understood.

Purpose of the Study:

  • To investigate the biodegradation potential of [14C]dimethylsilanediol in various soil types.
  • To identify microorganisms capable of degrading dimethylsilanediol and to develop methods for isolating them.
  • To explore the use of primary substrates for enhancing dimethylsilanediol cometabolism.

Main Methods:

  • Radiolabeling of dimethylsilanediol with Carbon-14 ([14C]) to track degradation.
  • Incubation of [14C]dimethylsilanediol in diverse soil samples and monitoring 14CO2 evolution.

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  • Development of a method to select for primary substrates supporting cometabolism.
  • Isolation and cultivation of microorganisms in liquid culture using selected substrates.
  • Identification of isolated microbial species (fungus: Fusarium oxysporum, bacterium: Arthrobacter sp.).

    • Main Results:

    • Dimethylsilanediol was biodegraded in all tested soils, evidenced by 14CO2 production.
    • Addition of 2-propanol as a carbon source significantly increased 14CO2 production, indicating enhanced cometabolism.
    • Fusarium oxysporum and an Arthrobacter species were isolated and confirmed to cometabolize dimethylsilanediol.
    • The isolated Arthrobacter species demonstrated the ability to utilize dimethylsulfone as a primary carbon source, a novel finding.

    Conclusions:

    • Dimethylsilanediol is biodegradable in soil environments.
    • Cometabolism, supported by suitable primary substrates, is an effective mechanism for dimethylsilanediol degradation.
    • Specific microbial species, including Fusarium oxysporum and Arthrobacter sp., play a role in breaking down dimethylsilanediol.
    • The discovery of a microorganism utilizing dimethylsulfone as a sole carbon source opens new avenues for bioremediation research.