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Engineering Bacillus thuringiensis bioinsecticides with an indigenous site-specific recombination system

J A Baum1, M Kakefuda, C Gawron-Burke

  • 1Ecogen, Inc., Langhorne, Pennsylvania 19047-3023, USA. jimbaum@voicenet.com

Applied and Environmental Microbiology
|December 1, 1996
PubMed
Summary

Bacillus thuringiensis (Bt) cry genes produce insecticidal proteins. A new gene transfer system enables genetic manipulation of Bt for enhanced biopesticides with improved insecticidal properties.

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Area of Science:

  • Microbiology
  • Molecular Biology
  • Biotechnology

Background:

  • Bacillus thuringiensis (Bt) produces crystal (Cry) proteins with insecticidal activity against various pests.
  • Improving the efficacy of Bt-based biopesticides is crucial for sustainable agriculture.
  • Genetic manipulation of Bt offers a pathway to enhance its insecticidal properties.

Purpose of the Study:

  • To develop a novel gene transfer system for Bacillus thuringiensis.
  • To enable the introduction and maintenance of insecticidal cry genes on plasmids.
  • To facilitate the engineering of Bt strains with improved insecticidal properties.

Main Methods:

  • Development of a gene transfer system using a Bt plasmid replicon.
  • Integration of an indigenous site-specific recombination system.

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  • Selective removal of ancillary DNA from recombinant Bt.

    • Main Results:

    • Successful introduction and maintenance of cloned insecticidal cry genes in Bt.
    • Demonstration of the vector system's ability to facilitate genetic manipulation.
    • Creation of engineered Bt strains with novel Cry gene combinations.

    Conclusions:

    • The developed gene transfer system is effective for manipulating Bt cry genes.
    • This system allows for the engineering of improved Bt-based biopesticides.
    • Site-specific recombination is a valuable tool for creating novel insecticidal agents.