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Mannitol, a novel bacterial compatible solute in Pseudomonas putida S12

E P Kets¹, E A Galinski, M de Wit

¹Division of Industrial Microbiology, Department of Food Science, Wageningen Agricultural University, The Netherlands. E.P.W.KETS@ATO.DLO.NL.

Journal of Bacteriology

|December 1, 1996

Summary

Pseudomonas putida S12 accumulates mannitol and NAGGN under osmotic stress. Mannitol, a polyol, plays a key role in the osmoadaptation of this bacterium.

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Area of Science:

Microbiology
Biochemistry

Background:

Bacteria like Pseudomonas putida S12 inhabit environments with fluctuating water availability.
Osmotic stress triggers cellular responses to maintain water homeostasis.
Compatible solutes are essential for microbial survival under hyperosmotic conditions.

Purpose of the Study:

To identify compatible solutes accumulated by Pseudomonas putida S12 under osmotic stress.
To elucidate the role of these solutes in bacterial osmoadaptation.

Main Methods:

Nuclear magnetic resonance (NMR) spectroscopy (13C and 1H) for solute identification.
Liquid chromatography-mass spectrometry (LC-MS) and high-performance liquid chromatography (HPLC) for quantification.
Controlled osmotic stress experiments with varying osmolytes and carbon sources.

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Main Results:

Pseudomonas putida S12 accumulated Nalpha-acetylglutaminylglutamine amide (NAGGN) and mannitol in response to osmotic stress.
Mannitol was identified as a novel compatible solute in this bacterium.
Solute accumulation varied with osmolyte type/concentration and carbon source.
Betaine addition led to its accumulation and depletion of NAGGN and mannitol.
Mannitol accumulation was observed in other Pseudomonas putida strains under salt stress.

Conclusions:

Mannitol is a key de novo-synthesized compatible solute for osmoadaptation in Pseudomonas putida S12.
This study highlights the principal role of the polyol mannitol in heterotrophic eubacterial osmoadaptation.
The findings expand our understanding of bacterial stress response mechanisms.