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Suppression of T lymphocyte proliferation by peritoneal macrophages in chickens
1Department of Veterinary Immunology, College of Agriculture, University of Osaka Prefecture, Japan.
Abstract:
The mechanism of the suppression of lymphocyte proliferation by peritoneal macrophages (MPs) in chickens and the presence of a suppressive substance in the culture supernatant of MPs were studied. The results showed that gelatin- and peptone-induced peritoneal MPs had an inhibitory accessory function against blastogenesis of mitogen-stimulated splenic lymphocytes and that resident peritoneal MPs had an enhanced accessory function. The suppressive factor produced by gelatin-induced peritoneal MPs is a protein having a pI of 3.6 and a molecular weight of 64,000 and it is physically and chemically a relatively stable substance. Moreover, it has no cytotoxic effect against lymphocytes and it was verified that the suppressor in the culture supernatant of MPs was not prostaglandin E2 or alpha-acid glycoprotein.
Insights
Chicken peritoneal macrophages (MPs) suppress lymphocyte proliferation. A stable, protein-based suppressive factor from MPs, distinct from prostaglandin E2, was identified, inhibiting lymphocyte blastogenesis.
Area of Science:
- Immunology
- Cell Biology
Background:
- Peritoneal macrophages (MPs) play a role in immune regulation.
- Understanding lymphocyte proliferation suppression is key to immune response.
Purpose of the Study:
- To investigate the mechanism of lymphocyte proliferation suppression by chicken MPs.
- To identify and characterize the suppressive substance in MP culture supernatant.
Main Methods:
- Studied the accessory function of resident and induced MPs on mitogen-stimulated splenic lymphocytes.
- Analyzed the culture supernatant of MPs for suppressive factors.
- Characterized the protein nature, pI, molecular weight, stability, and cytotoxic effects of the suppressor.
Main Results:
- Gelatin- and peptone-induced MPs exhibited inhibitory accessory function against lymphocyte blastogenesis.
- Resident MPs showed enhanced accessory function.
- A stable protein suppressor (pI 3.6, MW 64,000) was identified, lacking cytotoxic effects.
- The suppressor was confirmed not to be prostaglandin E2 or alpha-acid glycoprotein.
Conclusions:
- Chicken peritoneal macrophages (MPs) actively suppress lymphocyte proliferation.
- A specific protein factor produced by activated MPs mediates this suppression.
- This factor is distinct from known immunosuppressive molecules like prostaglandin E2.