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Plasma and cerebrospinal fluid hyperinsulinism in asphyxiated piglets
C S Abrahám1, P Temesvári, J Kovács
1Department of Pediatrics, Albert Szent-Györgyi Medical University, Szeged, Hungary.
Biology of the Neonate
|January 1, 1996
Summary
Neonatal asphyxia causes high insulin levels in both plasma and cerebrospinal fluid (CSF) in piglets. This hyperinsulinism may impact brain injury development after birth.
Area of Science:
- Neuroscience
- Endocrinology
- Perinatal Medicine
Background:
- Insulin is vital for perinatal brain development and may influence neonatal brain injury.
- Neonatal asphyxia is a critical event that can affect brain function and metabolism.
Purpose of the Study:
- To investigate the impact of neonatal asphyxia on insulin and glucose regulation in plasma and cerebrospinal fluid (CSF).
- To understand the changes in insulin and glucose metabolism during different phases of asphyxia and recovery in newborn piglets.
Main Methods:
- Utilized newborn piglets subjected to bilateral pneumothorax to induce asphyxia.
- Measured insulin concentrations via radioimmunoassay and glucose levels using the G oxidase method.
- Analyzed insulin and glucose levels in both plasma and CSF across basal, critical, and recovery phases.
Main Results:
- Observed significant hyperinsulinism (p < 0.001) and mild hypoglycemia (p < 0.05) in plasma and CSF during the recovery phase.
- Glucose infusion post-asphyxia exacerbated hyperinsulinism.
- The insulin/glucose ratio in CSF compared to plasma increased significantly 180 minutes after resuscitation (p < 0.05).
Conclusions:
- Neonatal asphyxia induces hyperinsulinism in both plasma and CSF.
- Altered insulin and glucose metabolism in the perinatal brain following asphyxia may contribute to hypoxic-ischemic brain damage.