Ramification and termination of single axons in the cerebellothalamic pathway of the rat

There is increasing speculation that individual neurones in the cerebellar nuclei are involved in the control of complex multi-joint movements rather than simple movements about a single-joint. These neurones project predominantly to the primary motor cortex after relaying in the motor thalamus. Given a) that localised regions of the motor cortex control individual muscles which generally act about single joints and b) the relatively tight topographical arrangement of thalamocortical connections, it is reasonable to hypothesise that if cerebellar output neurones control single-joint movements they are likely to project to localised areas of the motor thalamus, whereas if they project to more widespread regions they are likely to influence movements involving multiple joints. In this context, we have examined the ramifications and terminations of single anterogradely labelled axons in the cerebellothalamic pathway of the rat. A total of nine axons were traced (by using a 100 x oil objective) through serial sections from the caudal end of the thalamus to their terminations in the motor thalamus. Each of these axons gave off one or more collaterals which terminated in the intralaminar or other associated groups of thalamic nuclei, implying simultaneous activation of two functionally separate cerebellothalamic pathways. In the relay nucleus or motor thalamus, four axons formed either a single focal group of terminals or multiple groupings of terminals within a localised region, and five terminated over widespread regions including one which terminated bilaterally. These results show that a large proportion of cerebellar output neurones may be in a position to influence multi-joint or even bimanual movements.