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Updated: Aug 15, 2026

Multimer-PAGE: A Method for Capturing and Resolving Protein Complexes in Biological Samples
Published on: May 5, 2017
Capture in the gel: intermoleculare triplex formation during gel electrophoresis
B P Belotserkovskii1, B H Johnston
1Cell and Molecular Biology Laboratory, SRI International, Menlo Park, CA 94025, USA.
Abstract:
Analysis of unusual gel mobility patterns formed by certain DNA triplexes has revealed that intermolecular triplex formation can occur during gel electrophoresis when a faster migrating single strand overtakes a slower migrating band containing a duplex of appropriate sequence. Control experiments showed that this capture of the third strand occurs by sequence-specific hybridization rather than some nonspecific retardation. This phenomenon can be used to detect triplexes by a gel-shift assay even if their lifetime is much shorter than the time of gel electrophoresis.
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