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Related Experiment Videos

Expression and function of a CD4-like molecule in parathyroid tissue

P Hellman1, A Karlsson-Parra, L Klareskog

  • 1Department of Surgery, University Hospital, Uppsala, Sweden.

Surgery
|December 1, 1996
PubMed
Summary

The parathyroid chief cell expresses CD4, a molecule that regulates parathyroid hormone (PTH) release. This CD4 interaction in parathyroid tissue differs from its function in T-cells.

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Area of Science:

  • Endocrinology
  • Immunology
  • Cell Biology

Background:

  • Parathyroid tissue expresses T-lymphocyte antigens CD3 and CD4.
  • Parathyroid CD3 has been implicated in regulating parathyroid hormone (PTH) release.

Purpose of the Study:

  • To investigate the role of CD4 in parathyroid hormone secretion.
  • To determine if parathyroid CD4 is similar to lymphocytic CD4.
  • To compare PTH levels in healthy individuals and those with HIV-1.

Main Methods:

  • Immunohistochemistry using anti-Leu3a antibody to detect CD4 in parathyroid tissue.
  • Enzymatic dispersion of parathyroid cells to study PTH secretion.
  • Northern blot and PCR to analyze CD4 similarity.
  • Immunoradiometric assay for serum PTH measurement.

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Main Results:

  • Variable CD4 expression was observed in parathyroid tissue.
  • Parathyroid CD4 was identified as a 56 kd molecule similar to lymphocyte CD4.
  • Anti-Leu3a inhibited low calcium-stimulated PTH secretion without affecting intracellular calcium.
  • Individuals with HIV-1 had lower serum PTH levels than controls.

Conclusions:

  • Human parathyroid chief cells express a CD4 molecule involved in PTH release.
  • This parathyroid CD4 appears to utilize a different signaling pathway than T-cell CD4.
  • CD4 may play a role in both in vitro and in vivo regulation of PTH secretion.