Purification of protein C a natural anticoagulant, from human plasma by affinity chromatography with Convanavalin A
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E Pérez-Campos1, F Córdoba, E Pérez-Ortega
1Biochemistry and Immunology Unit, Technological Institute of Oaxaca, Mexico.
Preparative Biochemistry & Biotechnology
|August 1, 1996
Abstract:
We have developed a rapid, efficient, and inexpensive method to purify protein C from plasma using Concanavalin A conjugated to Sepharose. With this method we have obtained protein C purified to homogeneity, as measured in polyacrylamide gels. We discuss each of the steps of our method and give the results of the purification.
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