Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Preparative electrofocusing for industrial applications

R Hecht1, T Tressel, V Goldshteyn

  • 1Process Development Department, Amgen, Inc., Thousand Oaks, CA 91320-1789, USA.

Preparative Biochemistry & Biotechnology
|August 1, 1996
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Spectral shape of the two-photon decay of the 2 1S0 state in he-like tin.

Physical review letters·2010
Same author

The MAXEBIS at GSI as a test ion source for charge breeding and for HITRAP.

The Review of scientific instruments·2008
Same author

Current risk assessment approaches in different countries.

Food additives and contaminants·2000
Same author

Relationships between dioxins in soil, air, ash, and emissions from a municipal solid waste incinerator emitting large amounts of dioxins.

Chemosphere·1998
Same author

Efficacy of exogenous recombinant murine leptin in lean and obese 10- to 12-mo-old female CD-1 mice.

The American journal of physiology·1998
Same author

Pharmacoeconomic analysis of 3 treatment strategies for cytomegalovirus retinitis in patients with AIDS.

PharmacoEconomics·1998
Same journal

Bio-inspired rapid green chemical pretreatment for astaxanthin extraction utilizing shrimp waste: a Taguchi optimized scalable approach.

Preparative biochemistry & biotechnology·2026
Same journal

Biosynthesis of silver nanoparticles by endophytic <i>Aspergillus terreus</i>: Biomolecular capping in antioxidant, metal-chelating and genoprotective activities.

Preparative biochemistry & biotechnology·2026
Same journal

Green synthesis of ZnO nanoparticles using <i>Centroceras clavulatum</i> and evaluation of their multifunctional biological activities and cytotoxicity.

Preparative biochemistry & biotechnology·2026
Same journal

Strategic multistage optimization enhances bile salt hydrolase production in <i>Heyndrickxia coagulans</i> ATCC 7050 and <i>Lactiplantibacillus plantarum</i> ATCC 10012.

Preparative biochemistry & biotechnology·2026
Same journal

Black soybean waste as a sustainable substrate for γ-PGA production: comparative fermentation strategy and statistical process optimization.

Preparative biochemistry & biotechnology·2026
Same journal

Enhancing the stability of <i>Centella asiatica</i> bioactive compounds via ultrasound-assisted extraction and microencapsulation via ionotropic gelation.

Preparative biochemistry & biotechnology·2026
See all related articles

This study demonstrates a multicompartment isoelectric focusing apparatus for analyzing neurotrophic factors like brain-derived neurotrophic factor (BDNF) and neurotrophic factor 3 (NT3). The method accurately determines isoelectric points and preparative quantities, outperforming sequence-based predictions.

Area of Science:

  • Biochemistry
  • Protein Analysis
  • Analytical Chemistry

Background:

  • Isoelectric focusing (IEF) is a key technique in protein separation.
  • Accurate determination of isoelectric points (pI) is crucial for protein characterization.
  • Existing methods may have limitations in simultaneous analytical and preparative applications.

Purpose of the Study:

  • To evaluate a multicompartment isoelectric focusing apparatus for protein analysis.
  • To determine the isoelectric points and preparative quantities of BDNF and NT3.
  • To assess the separation of PEGylated and unPEGylated G-CSF forms.

Main Methods:

  • Utilized a multicompartment isoelectric focusing apparatus.
  • Applied the technique to brain-derived neurotrophic factor (BDNF) and neurotrophic factor 3 (NT3).

Related Experiment Videos

  • Investigated the separation of granulocyte colony-stimulating factor (G-CSF) with and without PEGylation.

    • Main Results:

    • BDNF and NT3 exhibited higher pI values than predicted by sequence-based modeling.
    • Successfully separated PEGylated from unPEGylated G-CSF.
    • Failed to separate proteins with differing degrees of PEGylation.

    Conclusions:

    • The multicompartment IEF apparatus is effective for determining pI and preparative quantities of specific neurotrophic factors.
    • The system offers a dual analytical and preparative function, potentially replacing separate IEF gels.
    • Further optimization is needed for separating proteins with varying PEGylation levels.