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Assay development for a portable fiberoptic biosensor

G P Anderson1, K A Breslin, F S Ligler

  • 1Center for Bio/Molecular Science and Engineering, Naval Research Laboratory, Washington, DC 20375-5348, USA.

ASAIO Journal (American Society for Artificial Internal Organs : 1992)
|November 1, 1996
PubMed
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A new portable fiberoptic biosensor offers rapid and sensitive detection of biologic analytes like Yersinia pestis and Bacillus anthracis antigens. This device improves detection limits by tenfold, enabling accurate on-site diagnostics with high specificity.

Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Immunosensing

Background:

  • Fiberoptic biosensors enable rapid and sensitive fluoroimmunoassays for biologic analytes.
  • Early laboratory devices showed promise but were impractical for on-site diagnostics due to size.
  • Matrix effects from biological samples like plasma and serum were minimal in initial assays.

Purpose of the Study:

  • To develop a portable, multichannel fiberoptic biosensor for on-site diagnostics.
  • To adapt existing fluoroimmunoassays for use with a new portable biosensor system.
  • To evaluate the performance of the portable biosensor for detecting specific bacterial antigens.

Main Methods:

  • Development of a portable, multichannel fiberoptic biosensor utilizing 635 nm laser diodes.

Related Experiment Videos

  • Conversion of fluoroimmunoassays to use cyanine dye (Cy5) labeled detection antibodies.
  • Testing the biosensor's ability to detect F1 antigen of Yersinia pestis and protective antigen of Bacillus anthracis.

    • Main Results:

    • Assays adapted for the portable biosensor showed a tenfold improvement in the limit of detection.
    • The portable biosensor achieved 100% detection for F1 antigen at concentrations of 100 ng/ml or higher.
    • The biosensor demonstrated 88% specificity in a blind test of serum samples spiked with F1 antigen.

    Conclusions:

    • The developed portable fiberoptic biosensor is effective for rapid and sensitive on-site detection of bacterial antigens.
    • The improved detection limits and high sensitivity make the biosensor suitable for clinical and diagnostic applications.
    • The biosensor's performance, including high sensitivity and specificity, supports its utility in field-based pathogen detection.