Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

An intronic polymorphic repeat sequence modulates interleukin-1 alpha gene regulation

S Bailly1, N Israël, M Fay

  • 1DBMS/BRCE-INSERM U244, CENG, Grenoble, France. S.Bailly@geant.ceng.cea.fr

Molecular Immunology
|August 1, 1996
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Sleep apnoea phenotypes in women: A cluster analysis from the ESADA cohort.

Sleep medicine·2024
Same author

Nivolumab in patients with advanced renal cell carcinoma in France: interim results of the observational, real-world WITNESS study.

ESMO open·2024
Same author

Impact of heterozygous ALK1 mutations on the transcriptomic response to BMP9 and BMP10 in endothelial cells from hereditary hemorrhagic telangiectasia and pulmonary arterial hypertension donors.

Angiogenesis·2024
Same author

Socioeconomic inequalities in cervical cancer mortality in Canada, 1990 and 2019: a trend analysis.

Public health·2024
Same author

Measles in vulnerable populations: An outbreak in Roma settlements of Loire-Atlantique, France, 2019.

Journal of medical virology·2023
Same author

Pediatric pulmonary arterial hypertension due to a novel homozygous GDF2 missense variant affecting BMP9 processing and activity.

American journal of medical genetics. Part A·2023
Same journal

Neutrophil elastase-SERPINB1 axis in epithelial responses: Cell death, inflammation and SERPINB1 dynamics.

Molecular immunology·2026
Same journal

Phillyrin alleviates pulmonary fibrosis via modulation of the TGF-β1/Smad and Nrf2/HO-1 signaling pathways.

Molecular immunology·2026
Same journal

m6A modification of LINC00458 enhances HMOX1 stability via ELAVL1 recruitment to promote ferroptosis and aggravate asthma.

Molecular immunology·2026
Same journal

Overexpression of Hes1 inhibits cigarette smoke-induced mitochondrial apoptosis in AT2 cells by activating the Pgc-1α/Tfam signaling pathway.

Molecular immunology·2026
Same journal

Progesterone promotes favorable pregnancy outcomes in recurrent spontaneous, abortion by attenuating NK Cell overactivation and upregulating the cAMP/PKA/CREB signaling axis.

Molecular immunology·2026
Same journal

Oleanolic acid alleviates hepatic fibrosis by inhibiting liver macrophage recruitment and polarization.

Molecular immunology·2026
See all related articles

A newly identified polymorphism in the Interleukin-1 alpha (IL-1 alpha) gene intron 6, characterized by variable tandem repeats, appears to negatively regulate IL-1 alpha gene transcription. Increased repeats correlate with decreased gene expression, suggesting a role in controlling IL-1 alpha levels.

Area of Science:

  • Genetics
  • Molecular Biology
  • Immunology

Background:

  • Interleukin-1 alpha (IL-1 alpha) is a key cytokine involved in inflammation and immune responses.
  • Genetic variations can influence gene expression and protein function.
  • A polymorphism in IL-1 alpha intron 6, featuring a variable number of tandem repeats (VNTR), was previously identified.

Purpose of the Study:

  • To investigate the functional impact of the IL-1 alpha intron 6 VNTR polymorphism on IL-1 alpha gene regulation.
  • To determine if the number of repeats influences IL-1 alpha gene transcription.
  • To explore the potential binding of transcription factors to the repeat sequence.

Main Methods:

  • Reporter gene assays were used to measure gene expression driven by the IL-1 alpha promoter with varying numbers of the repeat sequence.

Related Experiment Videos

  • Electrophoretic mobility shift assays (EMSAs) were employed to assess the binding of transcription factor Sp1 to the 46 bp repeat sequence.
  • In vitro IL-1 alpha production levels were measured in relation to the number of repeats.

    • Main Results:

    • Reporter gene expression decreased with an increasing number of tandem repeats in the IL-1 alpha intron 6 sequence.
    • The transcription factor Sp1 was shown to bind to the 46 bp repeat sequence.
    • No statistically significant correlation was found between IL-1 alpha production and the number of repeats, though a trend towards inverse relationship was observed.

    Conclusions:

    • The IL-1 alpha intron 6 repeat sequence exhibits a negative regulatory role on basal IL-1 alpha gene transcription.
    • The number of tandem repeats within this polymorphism may influence IL-1 alpha gene expression levels.
    • Sp1 binding to the repeat sequence could be a mechanism mediating this transcriptional regulation.