Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

An improved test system for PCR-based specific detection of Echinococcus multilocularis eggs

A Mathis1, P Deplazes, J Eckert

  • 1Institute of Parasitology, University of Zurich, Switzerland.

Journal of Helminthology
|September 1, 1996
PubMed
Summary

A new method enhances the detection of Echinococcus multilocularis eggs in fox feces using polymerase chain reaction (PCR). This technique offers high sensitivity and specificity for diagnosing parasite infections in wildlife.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Use of transesophageal echocardiography guidance for pulmonary artery banding in a cat with ventricular septal defect.

Journal of veterinary cardiology : the official journal of the European Society of Veterinary Cardiology·2026
Same author

Vector competence of Culicoides biting midges from Switzerland for African horse sickness virus and epizootic haemorrhagic disease virus.

Schweizer Archiv fur Tierheilkunde·2022
Same author

Cooperative Shear in Bulk Metallic Glass Composites Containing Metastable β-Ti Dendrites.

Physical review letters·2020
Same author

High pressure torsion induced lowering of Young's modulus in high strength TNZT alloy for bio-implant applications.

Journal of the mechanical behavior of biomedical materials·2020
Same author

Reference values and clinical predictors of bone strength for HR-pQCT-based distal radius and tibia strength assessments in women and men.

Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·2020
Same author

Twelve previously healthy non-geriatric dogs present for acute kidney injury after general anaesthesia for non-emergency surgical procedures in the UK.

The Journal of small animal practice·2020

Area of Science:

  • Veterinary Parasitology
  • Molecular Diagnostics
  • Wildlife Epidemiology

Background:

  • Echinococcus multilocularis is a significant parasite in canids, posing zoonotic risks.
  • Accurate detection of E. multilocularis eggs in fox feces is crucial for monitoring parasite prevalence and transmission.

Purpose of the Study:

  • To evaluate a novel method for sensitive detection of Echinococcus multilocularis eggs in fox feces using PCR.
  • To assess the specificity and sensitivity of the developed PCR assay.

Main Methods:

  • Concentration of helminth eggs from fox feces using sequential sieving (40 and 20 microns) and zinc chloride flotation.
  • DNA extraction via alkaline lysis from eggs retained in the 20-micron sieve.
  • Polymerase chain reaction (PCR) amplification using E. multilocularis species-specific primers.

Related Experiment Videos

Main Results:

  • The PCR method demonstrated 100% specificity and 94% sensitivity compared to intestinal examination.
  • False-negative results were associated with immature worm infections.
  • The PCR assay was robust, showing no inhibition with varying fecal volumes and tolerance to different sample preservation methods.

Conclusions:

  • The combined sieving, flotation, and PCR method provides a sensitive and specific tool for detecting E. multilocularis eggs in fox feces.
  • This diagnostic approach is valuable for epidemiological studies and wildlife health surveillance.
  • The method's adaptability to different sample conditions enhances its practical application.