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A TATA binding protein-associated factor functions as a coactivator for thyroid hormone receptors
K J Petty1, Y I Krimkevich, D Thomas
1Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas 75235-8857, USA. petty01@utsw.swmed.edu
Thyroid hormone receptors (TRs) interact with TATA-binding protein-associated factors (TAFs). TAFII110 acts as a cofactor for TRs, influencing thyroid hormone signaling and adding selectivity to hormonal responses.
Area of Science:
- Molecular Biology
- Endocrinology
- Gene Regulation
Background:
- Thyroid hormone receptors (TRs) mediate hormonal responses through protein interactions.
- TATA-binding protein-associated factors (TAFs) are known cofactors for various transcription factors.
Purpose of the Study:
- To investigate if TAFs serve as cofactors for TRs.
- To characterize the interaction between human TR beta and Drosophila TAFs.
Main Methods:
- In vitro protein interaction assays.
- Yeast two-hybrid system.
- Transfection assays.
Main Results:
- Human TR beta specifically bound to Drosophila TAFII110 and weakly to dTAFII60.
- The interaction between dTAFII110 and TR required their carboxyl-termini.
- dTAFII110 enhanced the transcriptional function of human TR beta.
Conclusions:
- TAFII110 functions as a cofactor for TRs.
- Specific TAF-nuclear receptor interactions provide selectivity in hormone signaling pathways.
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