Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Single-chain Fv radioimmunotargeting

J S Huston1, A J George, G P Adams

  • 1Creative BioMolecules, Inc., Department of Immunology, Royal Postgraduate Medical School, Hammersmith Hospital, London, United Kingdom.

The Quarterly Journal of Nuclear Medicine : Official Publication of the Italian Association of Nuclear Medicine (AIMN) [And] the International Association of Radiopharmacology (IAR)
|September 1, 1996
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Vulvar and vestibulovaginal hemangiosarcoma in a cow: morphological and histopathological observations.

Iranian journal of veterinary research·2023
Same author

Comparative assessment of faecal diagnostics for detection and predictive modelling of endemic Fasciola hepatica infection in sheep and cattle on Australian farms.

Veterinary parasitology·2021
Same author

Effectiveness of self-portraits used over personal protective equipment during the COVID-19 pandemic among patients and healthcare workers.

The British journal of surgery·2021
Same author

Comparative assessment of faecal diagnostics for detection and predictive modelling of endemic <i>Fasciola hepatica</i> infection in sheep and cattle on Australian farms.

Veterinary parasitology: X·2020
Same author

The convergence of regenerative medicine and rehabilitation: federal perspectives.

NPJ Regenerative medicine·2018
Same author

A co-twin control study of the association between combat exposure, PTSD and obesity in male veterans.

Psychological medicine·2018
Same journal

The role of labeled Annexin A5 in imaging of programmed cell death. From animal to clinical imaging.

The quarterly journal of nuclear medicine : official publication of the Italian Association of Nuclear Medicine (AIMN) [and] the International Association of Radiopharmacology (IAR)·2004
Same journal

Imaging cell death in vivo.

The quarterly journal of nuclear medicine : official publication of the Italian Association of Nuclear Medicine (AIMN) [and] the International Association of Radiopharmacology (IAR)·2004
Same journal

Radiolabelled peptides and low molecular weight proteins in metabolic diseases.

The quarterly journal of nuclear medicine : official publication of the Italian Association of Nuclear Medicine (AIMN) [and] the International Association of Radiopharmacology (IAR)·2004
Same journal

"Pathophysiologic mapping" of venous thromboembolism: opportunities for radiolabeled peptides.

The quarterly journal of nuclear medicine : official publication of the Italian Association of Nuclear Medicine (AIMN) [and] the International Association of Radiopharmacology (IAR)·2004
Same journal

Non-oncologic applications of radiolabeled peptides in nuclear medicine.

The quarterly journal of nuclear medicine : official publication of the Italian Association of Nuclear Medicine (AIMN) [and] the International Association of Radiopharmacology (IAR)·2004
Same journal

The role of octreotide scintigraphy in rheumatoid arthritis and sarcoidosis.

The quarterly journal of nuclear medicine : official publication of the Italian Association of Nuclear Medicine (AIMN) [and] the International Association of Radiopharmacology (IAR)·2004
See all related articles

Engineered single-chain variable fragment (sFv) proteins show promise for radioimmunotargeting. Optimizing administration routes and radiolabeling strategies enhances tumor targeting specificity for improved cancer imaging and therapy.

Area of Science:

  • Biotechnology
  • Radiopharmaceutical Science
  • Oncology

Background:

  • Engineered antibody fragments, specifically single-chain variable fragments (sFv), are advancing radioimmunotargeting applications.
  • sFv proteins, as monomers or multimers, offer rapid clearance and high targeting specificity within hours.

Purpose of the Study:

  • To compare intravenous (i.v.) and intraperitoneal (i.p.) administration of sFv for radioimmunotargeting.
  • To investigate methods for improving radioiodine retention and radiometal chelation in sFv for enhanced imaging and therapy.

Main Methods:

  • Comparison of i.v. versus i.p. administration of sFv in terms of biodistribution and targeting.
  • Evaluation of radioiodine dehalogenation resistance using para-131I-benzoyl conjugation.
  • Genetic modification of sFv to incorporate a cysteinyl peptide (sFv') for radiometal chelation (e.g., 99mTc, 186Re, 188Re).

Related Experiment Videos

Main Results:

  • Both i.v. and i.p. routes showed comparable long-term retention, but i.v. administration resulted in higher initial peak blood levels.
  • Repeated sFv injections achieved high target-to-background ratios, while continuous infusion led to elevated free sFv in blood and kidneys.
  • Resistant radioiodine labeling improved sFv localization in tumor xenografts; sFv' facilitated radiometal incorporation for diagnostic and therapeutic potential.

Conclusions:

  • Administration route and radiolabeling strategy significantly impact sFv biodistribution and tumor targeting.
  • Engineered sFv proteins, particularly sFv', hold potential for advanced cancer diagnostics and therapeutics through radioimmunotargeting.