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Related Experiment Videos

Conserved circadian elements in phylogenetically diverse algae

M Mittag1

  • 1Botanisches Institut, Ludwig-Maximilians-Universität München, Germany. mittag@botanik.biologie.uni-muenchen.de

Proceedings of the National Academy of Sciences of the United States of America
|December 10, 1996
PubMed
Summary
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Circadian regulation of gene expression involves conserved elements. A protein analog to Gonyaulax polyedra's circadian clock-controlled trans-acting factor (CCTR) was identified in Chlamydomonas reinhardtii, binding to UG-repeats.

Area of Science:

  • Molecular Biology
  • Chronobiology
  • Algal Biology

Background:

  • Circadian expression of luciferin-binding protein (LBP) in Gonyaulax polyedra is translationally controlled.
  • A specific 3'-UTR interval with UG-repeats in the LBP gene acts as a cis-acting element.
  • This element binds a circadian-regulated trans-acting factor (CCTR) negatively impacting LBP expression.

Purpose of the Study:

  • To identify a potential CCTR analog in the green alga Chlamydomonas reinhardtii.
  • To investigate the conservation of circadian translational regulatory elements across different species.
  • To confirm the binding of this analog to the UG-repeat region under circadian control.

Main Methods:

  • Protein identification and characterization in Chlamydomonas reinhardtii.

Related Experiment Videos

  • Electrophoretic mobility shift assays (EMSAs) to assess protein-DNA binding.
  • Analysis of binding activity in relation to the circadian clock.

    • Main Results:

    • A protein in Chlamydomonas reinhardtii was identified as a CCTR analog.
    • This analog specifically binds to the UG-repeat region of the LBP gene.
    • The binding is under the control of the circadian clock.

    Conclusions:

    • Circadian cis-regulatory elements involved in translational control are evolutionarily conserved.
    • This finding provides evidence for conserved mechanisms in circadian gene regulation between dinoflagellates and green algae.
    • The identified CCTR analog offers a model for studying conserved translational regulation in eukaryotes.