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Separation of chiral polychlorinated biphenyls by micellar electrokinetic chromatography using beta- and
M L Marina1, I Benito, J C Díez-Masa
1Departamento de Química Analítica, Facultad de Ciencias, Universidad de Alcalá de Henares, Madrid, Spain.
Abstract:
Chiral polychlorinated biphenyls (PCBs) 45, 84, 88, 91, 95, 132, 136, 139, 149, 171, 183 and 196 were separated each in its two enantiomers by cyclodextrin-modified micellar electrokinetic chromatography (CD-MEKC). Mixtures of beta- and gamma-cyclodextrins were used as chiral modifiers in a 2-(N-cyclohexylamino)ethanesulphonic acid (CHES) buffer containing urea and sodium dodecyl sulphate (SDS) micelles. Separations of multicomponent mixtures of PCBs into their enantiomers were also performed. A mixture of PCBs 45, 88, 91, 95, 136, 139, 149 and 196 was separated into all sixteen enantiomers in an analysis time of approx. 35 min.