[Fe-containing superoxide dismutase from Pseudomonas aeruginosa]

A Fe-containing superoxide dismutase (EC 1.15.1.1) has been isolated from the soil denitrifying bacterium Pseudomonas aeruginosa and characterized. An apoprotein has been obtained and protein reconstruction by Fe ions carried out. The absorbance in the visible part of the spectrum is fully determined by the Fe-containing cluster which does not influence the absorption in the UV region. The effects of inorganic anions on the enzyme activity and ESR spectra have been studied. The mechanisms of inhibition have been found to be different for F- and CNS-. Based on the data on the pH dependence of the shape of ESR spectra it is assumed that the environment of Fe-containing centers in the protein molecule is different.