[Luminescent analysis of peripheral blood lymphocytes in subjects engaged in production hazardous in reference to...

The authors demonstrate the possibility of using the fluorescent analysis of the peripheral blood lymphocytes for the detection of the specific sensitization of the body to allergens and of immune deficiency. A positive correlation has been revealed between intensified fluorescence (at 640 nm) of blood lymphocytes stained with fluorochrome acridine orange, in vitro stimulated with a specific allergen, and the blood serum antihapten humoral antibodies, as well as deviations of some other laboratory parameters from the norm. High informative value of the fluorescent analysis, use of small quantities of blood recommend this method for screening examinations and formation of groups at risk of developing occupational diseases.