[Analytical characteristics of commercial IRMA-AFP kit for determination of human alpha-fetoprotein]

Commercial polyclonal IRMA-AFP kit is manufactured by Radiopreparat in Tashkent since 1986. The kit has a range of 0 to 320 ng/ml. This report includes the data on AFP measurements in the sera of cancer patients, particularly at the lower part of the calibration curve. Within run precision studies yielded a CV of 7.7% at the 4.3-54.0 ng/ml interval. Between run precision was 8.6% at the 3.3-56.0 ng/ml interval. The recovery of AFP in the presence of matrix effects of 9 sera was 98% for mixtures at the 9.1-60.8 ng/ml range. The assay sensitivity was 0.6 ng/ml. In donor blood samples the AFP levels did not exceed 4 ng/ml.