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[Differentiation of Mycobacteria]

G K Kovalev

    Klinicheskaia Laboratornaia Diagnostika
    |May 1, 1993
    PubMed
    Summary

    Enzyme activities like lipase, phosphatase, and aryl sulfatase help differentiate mycobacterial species. This study analyzed 387 cultures, revealing distinct patterns for various mycobacteria groups.

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    Area of Science:

    • Microbiology
    • Enzymology

    Background:

    • Mycobacterial species identification is crucial for clinical and epidemiological purposes.
    • Enzyme activity profiling offers a potential method for bacterial differentiation.

    Purpose of the Study:

    • To investigate the lipase, phosphatase, and aryl sulfatase activities in 387 mycobacterial cultures.
    • To assess the utility of these enzyme activities for differentiating various mycobacterial species and groups.

    Main Methods:

    • Culturing of 387 mycobacterial isolates from diverse species and Ragnion groups.
    • Assay of lipase, phosphatase, and aryl sulfatase activities for each isolate.

    Main Results:

    • Phosphatase activity was detected in several species including M. tuberculosis and M. avium, with heterogeneous results in Group II Mycobacteria.
    • Lipase activity was prominent in M. phlei, M. smegmatis, and M. fortuitum, but absent in M. bovis and M. avium.
    • Aryl sulfatase activity was observed only in M. fortuitum and select cultures from M. scrofulaceum, M. marinum, M. avium, M. intracellulare, and Group II Mycobacteria.

    Conclusions:

    • Lipase, phosphatase, and aryl sulfatase activities exhibit varied patterns across different mycobacterial species.
    • These enzymatic profiles can aid in the differentiation and identification of mycobacteria.
    • Further investigation into specific enzyme markers may enhance mycobacterial taxonomy.

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