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Cloning and sequence analysis of the human salivary peroxidase-encoding cDNA
C Kiser1, C K Caterina, J A Engler
1Department of Pediatric Dentistry, University of Alabama Schools of Medicine and Dentistry, Birmingham, AL 35294, USA.
Gene
|September 16, 1996
Abstract:
A cDNA (hSPO) encoding human salivary peroxidase (hSPO) was isolated and its nucleotide (nt) sequence determined. The coding sequence resembles those of other mammalian peroxidases and contains a 3' untranslated region of 538 nt. In a rabbit reticulocyte lysate transcription/translation system, the cDNA produces a major protein of approx. 67 kDa, which corresponds to the calculated molecular weight of unglycosylated hSPO.